Total synthesis of cytochrome b562 by native chemical ligatin using a removable auxiliary

Total synthesis of cytochrome b562 by native chemical ligatin using a removable auxiliary

We have completed the total chemical synthesis of cytochrome b562 and an axial ligand analogue, [SeMet(7)]cyt b562, by thioestermediated chemical ligation of unprotected peptide segments. A novel auxilliary-mediated native chemical ligation that enables peptide ligation to be applied to protein sequ...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2001-06, Vol.98 (12), p.6554
Hauptverfasser: Low, Donald W, Hill, Michael G, Carrasco, Michael R, Kent, Stephen B H, Botti, Paolo
Format: Artikel
Sprache:eng
Schlagworte:
Chemistry
Peptides
Proteins
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Zusammenfassung:We have completed the total chemical synthesis of cytochrome b562 and an axial ligand analogue, [SeMet(7)]cyt b562, by thioestermediated chemical ligation of unprotected peptide segments. A novel auxilliary-mediated native chemical ligation that enables peptide ligation to be applied to protein sequences lacking cysteine was used.
ISSN:0027-8424
1091-6490