Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antiobodies designed with metal-binding domains

Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antiobodies designed with metal-binding domains

To study the molecular structure and function of gene products in situ, we developed a molecular immunolabeling technology. Starting with cDNA from hybridomas producing monoclonal antibodies against biotin, catalase, and superoxide dismutase, we bioengineered recombinant single-chain variable fragme...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2002-01, Vol.99 (1), p.213
Hauptverfasser: Malecki, Marek, Hsu, Annie, Truong, Lynn, Sanchez, Sylvia
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Cellular biology
Genetics
Molecules
Studies
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creator Malecki, Marek
Hsu, Annie
Truong, Lynn
Sanchez, Sylvia
description To study the molecular structure and function of gene products in situ, we developed a molecular immunolabeling technology. Starting with cDNA from hybridomas producing monoclonal antibodies against biotin, catalase, and superoxide dismutase, we bioengineered recombinant single-chain variable fragment antibodies (scFv) and their derivatives containing metal-binding domains (scFv:MBD).
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source Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects Cellular biology
Genetics
Molecules
Studies
title Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antiobodies designed with metal-binding domains
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