Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antiobodies designed with metal-binding domains

Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antiobodies designed with metal-binding domains

To study the molecular structure and function of gene products in situ, we developed a molecular immunolabeling technology. Starting with cDNA from hybridomas producing monoclonal antibodies against biotin, catalase, and superoxide dismutase, we bioengineered recombinant single-chain variable fragme...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2002-01, Vol.99 (1), p.213
Hauptverfasser: Malecki, Marek, Hsu, Annie, Truong, Lynn, Sanchez, Sylvia
Format: Artikel
Sprache:eng
Schlagworte:
Cellular biology
Genetics
Molecules
Studies
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:To study the molecular structure and function of gene products in situ, we developed a molecular immunolabeling technology. Starting with cDNA from hybridomas producing monoclonal antibodies against biotin, catalase, and superoxide dismutase, we bioengineered recombinant single-chain variable fragment antibodies (scFv) and their derivatives containing metal-binding domains (scFv:MBD).
ISSN:0027-8424
1091-6490