Partial molar volume, surface area, and hydration changes for equilibrium unfolding and formation of aggregation transition state: High-pressure and cosolute studies

Partial molar volume, surface area, and hydration changes for equilibrium unfolding and formation of aggregation transition state: High-pressure and cosolute studies

The equilibrium dissociation of recombinant human IFN-gamma was monitored as a function of pressure and sucrose concentration. The partial molar volume change for dissociation was -209 +/- 13 ml/mol of dimer. The specific molar surface area change for dissociation was 12.7 +/- 1.6 nm2/molecule of di...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2001-06, Vol.98 (13), p.7259
Hauptverfasser: Webb, Jonathan N, Webb, Serena D, Cleland, Jeffrey L, Carpenter, John F, Randolph, Theodore W
Format: Artikel
Sprache:eng
Schlagworte:
Biology
Molecules
Physics
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Zusammenfassung:The equilibrium dissociation of recombinant human IFN-gamma was monitored as a function of pressure and sucrose concentration. The partial molar volume change for dissociation was -209 +/- 13 ml/mol of dimer. The specific molar surface area change for dissociation was 12.7 +/- 1.6 nm2/molecule of dimer.
ISSN:0027-8424
1091-6490