Catalytic properties of selenophospate synthetases: Comparison of the selenocysteine-containing enzyme from Haemophilus influenzae with the corresponding cysteine-containing enzyme from Escherichia coli

The selD gene from Haemophilus influenzae has been overexpressed in Escherichia coli. The expressed protein was purified to homogeneity in a four-step procedure and then carboxymethylated by reaction with chloroacetate.

Bibliographische Detailangaben

Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1999-01, Vol.96 (1), p.44
Hauptverfasser:	Lacourciere, Gerard M, Stadtman, Thressa C
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biochemistry Enzymes Genes Influenza Proteins
Online-Zugang:	Volltext
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