Catalytic properties of selenophospate synthetases: Comparison of the selenocysteine-containing enzyme from Haemophilus influenzae with the corresponding cysteine-containing enzyme from Escherichia coli

Catalytic properties of selenophospate synthetases: Comparison of the selenocysteine-containing enzyme from Haemophilus influenzae with the corresponding cysteine-containing enzyme from Escherichia coli

The selD gene from Haemophilus influenzae has been overexpressed in Escherichia coli. The expressed protein was purified to homogeneity in a four-step procedure and then carboxymethylated by reaction with chloroacetate.

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1999-01, Vol.96 (1), p.44
Hauptverfasser: Lacourciere, Gerard M, Stadtman, Thressa C
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria
Biochemistry
Enzymes
Genes
Influenza
Proteins
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Zusammenfassung:The selD gene from Haemophilus influenzae has been overexpressed in Escherichia coli. The expressed protein was purified to homogeneity in a four-step procedure and then carboxymethylated by reaction with chloroacetate.
ISSN:0027-8424
1091-6490