Lipid Patches in Membrane Protein Oligomers: Crystal Structure of the Bacteriorhodopsin-Lipid Complex

Heterogenous nucleation on small molecule crystals causes a monoclinic crystal form of bacteriorhodopsin (BR) in which trimers of this membrane protein pack differently than in native purple membranes. Analysis of single crystals by nano-electrospray ionization-mass spectrometry demonstrated a prese...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1998-09, Vol.95 (20), p.11673-11678
Hauptverfasser: L.-O. Essen, Siegert, R., Lehmann, W. D., Oesterhelt, D.
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container_issue 20
container_start_page 11673
container_title Proceedings of the National Academy of Sciences - PNAS
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creator L.-O. Essen
Siegert, R.
Lehmann, W. D.
Oesterhelt, D.
description Heterogenous nucleation on small molecule crystals causes a monoclinic crystal form of bacteriorhodopsin (BR) in which trimers of this membrane protein pack differently than in native purple membranes. Analysis of single crystals by nano-electrospray ionization-mass spectrometry demonstrated a preservation of the purple membrane lipid composition in these BR crystals. The 2.9- angstrom x-ray structure shows a lipid-mediated stabilization of BR trimers where the glycolipid S-TGA-1 binds into the central compartment of BR trimers. The BR trimer/lipid complex provides an example of local membrane thinning as the lipid head-group boundary of the central lipid patch is shifted by 5 angstrom toward the membrane center. Nonbiased electron density maps reveal structural differences to previously reported BR structures, especially for the cytosolic EF loop and the proton exit pathway. The terminal proton release complex now comprises an E194-E204 dyad as a diffuse proton buffer.
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source Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects Bacteria
Biological Sciences
Biology
Crystals
Dyadic relations
Lipids
Membrane proteins
Membranes
Molecules
Monomers
Nucleation
P branes
Physics
Protons
Trimers
title Lipid Patches in Membrane Protein Oligomers: Crystal Structure of the Bacteriorhodopsin-Lipid Complex
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