Recombinant tobacco mosaic virus movement protein is an RNA-binding, (alpha)-helical membrane protein

Recombinant tobacco mosaic virus movement protein is an RNA-binding, (alpha)-helical membrane protein

The 30-kDa movement protein (MP) is essential for cell-cell spread of tobacco mosaic virus in planta. To explore the structural properties of MP, the full-length recombinant MP gene was expressed in Escherichia coli, and one-step purification from solubilized inclusion bodies was accomplished by usi...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2000-06, Vol.97 (13), p.7112
Hauptverfasser: Brill, Laurence M, Nunn, Rashmi S, Kahn, Theodore W, Yeager, Mark, Beachy, Roger N
Format: Artikel
Sprache:eng
Schlagworte:
Proteins
Ribonucleic acid
RNA
Tobacco
Viruses
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Zusammenfassung:The 30-kDa movement protein (MP) is essential for cell-cell spread of tobacco mosaic virus in planta. To explore the structural properties of MP, the full-length recombinant MP gene was expressed in Escherichia coli, and one-step purification from solubilized inclusion bodies was accomplished by using anion exchange chromatography.
ISSN:0027-8424
1091-6490