Affinity functionalization of PVA- co -PE nanofibrous membrane with Ni( ii )-chelated ligand for bovine hemoglobin adsorption

We report that poly(vinyl alcohol- co -ethylene) (PVA- co -PE) nanofibrous membrane was surface functionalized by iminodiacetic acid (IDA) and then chelated by Ni( ii ) ions for hemoglobin adsorption. The results indicated that bovine hemoglobin (BHb) adsorption on Ni( ii )-chelated PVA- co -PE nanofibrous membrane was better described by the Langmuir model. The experimental maximum adsorption capacity value was 66 mg g −1 . The method performed in this study provides an exploratory research for the large-scale purification of BHb and other proteins.