Rapid gene-specific repair of cisplatin lesions at the human DUG/DHFR locus comprising the divergent upstream gene and dihydrofolate reductase gene during early G(1) phase of the cell cycle assayed by using the exonucleolytic activity of T4 DNA polymerase

Rapid gene-specific repair of cisplatin lesions at the human DUG/DHFR locus comprising the divergent upstream gene and dihydrofolate reductase gene during early G(1) phase of the cell cycle assayed by using the exonucleolytic activity of T4 DNA polymerase

A novel assay to detect strand-specific DNA repair after cellular exposure to cisplatin at IC50 levels is used to measure rapid repair in the divergent upstream gene (DUG), a human MutS homolog, and in the bidirectional promoter for dihydrofolate reductase gene (DHFR) and the contiguous upstream DUG...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1994-11, Vol.91 (23), p.10977
Hauptverfasser: Rampino, Nicholas J, Bohr, Vilhelm A
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Biochemistry
Deoxyribonucleic acid
DNA
Genes
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Rampino, Nicholas J
Bohr, Vilhelm A
description A novel assay to detect strand-specific DNA repair after cellular exposure to cisplatin at IC50 levels is used to measure rapid repair in the divergent upstream gene (DUG), a human MutS homolog, and in the bidirectional promoter for dihydrofolate reductase gene (DHFR) and the contiguous upstream DUG.
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source Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects Biochemistry
Deoxyribonucleic acid
DNA
Genes
title Rapid gene-specific repair of cisplatin lesions at the human DUG/DHFR locus comprising the divergent upstream gene and dihydrofolate reductase gene during early G(1) phase of the cell cycle assayed by using the exonucleolytic activity of T4 DNA polymerase
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