Rapid gene-specific repair of cisplatin lesions at the human DUG/DHFR locus comprising the divergent upstream gene and dihydrofolate reductase gene during early G(1) phase of the cell cycle assayed by using the exonucleolytic activity of T4 DNA polymerase

A novel assay to detect strand-specific DNA repair after cellular exposure to cisplatin at IC50 levels is used to measure rapid repair in the divergent upstream gene (DUG), a human MutS homolog, and in the bidirectional promoter for dihydrofolate reductase gene (DHFR) and the contiguous upstream DUG.