RECA-DEPENDENT RECOVERY OF ARRESTED DNA REPLICATION FORKS
DNA damage encountered during the cellular process of chromosomal replication can disrupt the replication machinery and result in mutagenesis or lethality. The RecA protein of Escherichia coli is essential for survival in this situation: It maintains the integrity of the arrested replication fork an...
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Veröffentlicht in: | Annual review of genetics 2003-01, Vol.37 (1), p.611-646 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | DNA damage encountered during the cellular process of chromosomal
replication can disrupt the replication machinery and result in mutagenesis or
lethality. The RecA protein of
Escherichia coli
is essential for
survival in this situation: It maintains the integrity of the arrested
replication fork and signals the upregulation of over 40 gene products, of
which most are required to restore the genomic template and to facilitate the
resumption of processive replication. Although RecA was originally discovered
as a gene product that was required to change the genetic information during
sexual cell cycles, over three decades of research have revealed that it is
also the key enzyme required to maintain the genetic information when DNA
damage is encountered during replication in asexual cell cycles. In this
review, we examine the significant experimental approaches that have led to our
current understanding of the RecA-mediated processes that restore replication
following encounters with DNA damage. |
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ISSN: | 0066-4197 1545-2948 |
DOI: | 10.1146/annurev.genet.37.110801.142616 |