Nutrition content of sea urchin (Diadema setosum) gonad and antibacteria activities of its shell extracts

Diadema setosum is one species of sea urchins that have economic value. The body part of sea urchins that is commonly consumed is its gonad. This organism can be used as a source of nutritious food because it contains amino acids, vitamin B complex, vitamin A, minerals, omega-3 and omega-6 fatty acids. The shell of sea urchins also known as an antibacterial agent. This study aims to determine the nutritional content of D. setosum gonad and antibacterial activity of D. setosum shells extract. The sample of D. setosum gonad was collected from Liang, Waai and Sila Village waters in Central Maluku Regency. Whereas, the shells was derived from the sea urchin from Sila Village. The nutritional contents of sea urchin gonad were determined by a proximate analysis with AOAC method. The antibacterial activity was tested against methanol, ethyl acetate and chloroform extract of sea urchin shell through agar diffusion method. The results showed that the gonad of D. setosum from the three study sites contained of water content (72.24±0.02; 71.95±0.05 and 77.56±0.02)%, ashes (11.7±0.09; 7.34±0.14 and 11.32±0.04)%, db, lipid (3.21±0.20; 3.49±0.12 and 10.51±0.07)%, db and protein ( 44.8±0.36; 47.66±0.21 and 64.93±0.96)%, db. The shell extract showed the antibacterial effect against Escherichia coli, Salmonella sp. and Bacillus cereus the inhibition zone of each tested bacterial was different, as shown in methanol extract (1.84±0.05 mm; 1.84±0.03 mm and 2.65±0.02 mm), ethyl acetate extract (14.18±0.02 mm; 1.65±0.03 mm and 14.49±0.03 mm) and chloroform extract (0.64±0.08 mm; 8.98±0.03 mm and 3.77±0.14 mm).