Development of an immunoblot assay for carcinoembryonic antigen (CEA) in human serum using a portable UV illuminator

Development of an immunoblot assay for carcinoembryonic antigen (CEA) in human serum using a portable UV illuminator

A rapid quantum dot (QD) based immunoblot assay is developed to detect CEA in human serum using a portable UV illuminator. Here, photostable QD-conjugated antibodies were utilized that bind CEA in human serum, previously blotted on a nitrocellulose membrane for point-of-care applications.

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Veröffentlicht in:Analytical methods 2018-01, Vol.10 (9), p.947-949
Hauptverfasser: Chouhan, Raghuraj S., Niazi, Javed H., Qureshi, Anjum
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies
Carcinoembryonic antigen
Cellulose esters
Cellulose nitrate
Quantum dots
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Zusammenfassung:A rapid quantum dot (QD) based immunoblot assay is developed to detect CEA in human serum using a portable UV illuminator. Here, photostable QD-conjugated antibodies were utilized that bind CEA in human serum, previously blotted on a nitrocellulose membrane for point-of-care applications.
ISSN:1759-9660
1759-9679
DOI:10.1039/C7AY02929B