Reactivity of potassium permanganate and tetraethylammonium chloride with mismatchedbases and a simple mutation detection protocol

Reactivity of potassium permanganate and tetraethylammonium chloride with mismatchedbases and a simple mutation detection protocol

Many mutation detection techniques rely upon recog-nition of mismatched base pairs in DNA hetero-duplexes. Potassium permanganate in combination with tetraethylammonium chloride (TEAC) is capable of chemically modifying mismatched thymidine residues. The DNA strand can then be cleaved at that point...

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Veröffentlicht in:Nucleic acids research 1999-04, Vol.27 (8), p.1866
Hauptverfasser: Lambrinakos, A, Humphrey, K E, Babon, J J, Ellis, T P, Cotton, R G H
Format: Artikel
Sprache:eng
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Zusammenfassung:Many mutation detection techniques rely upon recog-nition of mismatched base pairs in DNA hetero-duplexes. Potassium permanganate in combination with tetraethylammonium chloride (TEAC) is capable of chemically modifying mismatched thymidine residues. The DNA strand can then be cleaved at that point by treatment with piperidine. The reactivity of potassium permanganate (KMnO
ISSN:0305-1048
1362-4962