Mercury removal by engineered Escherichia coli cells expressing different rice metallothionein isoforms
Mercury is one of the more common and potentially most harmful toxic metals. Remediation using conventional physical and chemical methods is uneconomical and generates large volumes of chemical waste. Bioremediation of hazardous metals has received considerable and growing interest over the years. I...
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Veröffentlicht in: | Annals of microbiology 2018-03, Vol.68 (3), p.145-152 |
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Sprache: | eng |
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Zusammenfassung: | Mercury is one of the more common and potentially most harmful toxic metals. Remediation using conventional physical and chemical methods is uneconomical and generates large volumes of chemical waste. Bioremediation of hazardous metals has received considerable and growing interest over the years. In the present work, genetically engineered
Escherichia coli
cells, which express four rice metallothionein (MT) isoforms as fusions with glutathione-S-transferase (GST), were tested for their ability to remove mercury. The results showed that the
E. coli
cells expressing OsMT1, OsMT2, OsMT3
,
and OsMT4 are able to remove 20, 13.7, 10
,
and 7 nmol Hg
2+
/mg (dry weight) from the culture medium, respectively. The recombinant GST
–
OsMTs were purified using affinity chromatography. The UV absorption spectra and the results of 5,5-dithio-bis-(2-nitrobenzoic) acid (DTNB) assay recorded after the reconstitution of the apo-OsMTs with mercury confirmed that the different OsMT isoforms were able to form mercury complexes in vitro with different binding capacities and different binding strength. |
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ISSN: | 1590-4261 1869-2044 |
DOI: | 10.1007/s13213-018-1326-2 |