Effects of puerarin combined with edaravone on inhalation lung injury induced by black gunpowder smog in rats

Objective To explore the protective effects of puerarin combined with edaravone on inhalation lung injury induced by black gunpowder smog in rats. Methods Forty healthy male Wistar rats were randomly divided into normal control group (group N), inhalation group (group X), puerarin group (group P), edaravone group (group E) and edaravone combined with puerarin group (group L), with 8 rats in each group. Rat model of inhalation lung injury was reproduced by a self-made smoke generator. Rats in group E were given intraperitoneal injections of edaravone (9 mg/kg) at 30 minutes and 1 day after modeling (twice totally). Rats in group P were given intraperitoneal injections of puerarin (100 mg/kg) at 30 minutes and 1, 2, 3, 4, 5 days after modeling (6 times totally). Rats in group L were treated the way of both group E and P. The rats in group N and group X were given intraperitoneal injections of normal saline (12 ml/kg) at the time-points above. The animals were sacrificed 6 days after modeling, and the blood samp