Culture and identification of stem cells of uterine junctional zone

Objective To establish a method for isolation, culture, and identification of stem cells in uterine junctional zone (uJZSCs). Methods Specimens of uterus muscle layer at uterine junctional zone (uJZ) were harvested under aseptic condition, and they digested, cultivated and amplified using trypsogen and collagenaseⅠ. The morphology of uJZSCs was observed with inverted microscope, and cell viability and phenotype were analyzed by flow cytometry. The adipogenic, osteogenic and chondrogenic differentiation was induced in vitro, and their biological growth characteristic was identified by CCK-8 marking. Results The cells were adhered after passage, and they presented long spindle in shape. Flow cytometry showed that the expressions of CD90, CD73, CD105, CD29, CD44, CD13, CD166, and HLA-ABC marking was also positive, while they were negative for CD34, CD45, CD14, HLA-DR and CD19. The CCK-8 growth curve was S-shaped. The induced differentiation experiments indicated that uJZSCs could be induced into osteoblast, adip