Advanced oxidation protein products induce apoptosis of HaCaT cells: an experimental study

Advanced oxidation protein products induce apoptosis of HaCaT cells: an experimental study

Objective 　To investigate the effect and mechanism of advanced oxidation protein products (AOPPs) on the apoptosis of HaCaT cells. Methods 　AOPPs were prepared by incubation of human serum albumin (HSA) with sodium hypochlorite solution. MTT assay was used to determine the effects of different conce...

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Veröffentlicht in:Jie fang jun yi xue za zhi 2016-05, Vol.41 (5), p.373
Hauptverfasser: Bai-hui, SUN, LI, Qin, Ruo-ting DING, Wen-lin, YU, Yan-hong, WU
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Apoptosis
Proteins
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container_issue 5
container_start_page 373
container_title Jie fang jun yi xue za zhi
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creator Bai-hui, SUN
LI, Qin
Ruo-ting DING
Wen-lin, YU
Yan-hong, WU
description Objective 　To investigate the effect and mechanism of advanced oxidation protein products (AOPPs) on the apoptosis of HaCaT cells. Methods 　AOPPs were prepared by incubation of human serum albumin (HSA) with sodium hypochlorite solution. MTT assay was used to determine the effects of different concentrations (0, 50, 100, 200 and 400µg/ml) of AOPPs on HaCaT cell activity by incubating the cells for 24h for selecting optimum working concentration of AOPPs. HaCaT cells were treated based on time effect or concentration effect. The incidence of cell apoptosis was measured by flow cytometry. Intracellular reactive oxygen species (ROS) production was determined with dichlorofluorescein diacetate (DCFH-DA). And the protein expressions of NOX4, Bax and Bcl-2 were examined by Western blotting. Results 　Viability of HaCaT cells treated with 400µg/ml AOPPs was significantly lowered (P
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Methods 　AOPPs were prepared by incubation of human serum albumin (HSA) with sodium hypochlorite solution. MTT assay was used to determine the effects of different concentrations (0, 50, 100, 200 and 400µg/ml) of AOPPs on HaCaT cell activity by incubating the cells for 24h for selecting optimum working concentration of AOPPs. HaCaT cells were treated based on time effect or concentration effect. The incidence of cell apoptosis was measured by flow cytometry. Intracellular reactive oxygen species (ROS) production was determined with dichlorofluorescein diacetate (DCFH-DA). And the protein expressions of NOX4, Bax and Bcl-2 were examined by Western blotting. Results 　Viability of HaCaT cells treated with 400µg/ml AOPPs was significantly lowered (P&lt;0.05). AOPPs, in a time-and concentration-dependent manner, induced the apoptosis of HaCaT cells (P&lt;0.05), increased the expression of NO</description><identifier>ISSN: 0577-7402</identifier><identifier>DOI: 10.11855/j.issn.0577-7402.2016.05.05</identifier><language>chi</language><publisher>Beijing: People's Military Medical Press</publisher><subject>Apoptosis ; Proteins</subject><ispartof>Jie fang jun yi xue za zhi, 2016-05, Vol.41 (5), p.373</ispartof><rights>2016] This work is licensed under [CC BY 3.0 Unported - https://creativecommons.org/licenses/by/3.0/ (“the License”). 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Proteins
title Advanced oxidation protein products induce apoptosis of HaCaT cells: an experimental study
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