Advanced oxidation protein products induce apoptosis of HaCaT cells: an experimental study

Objective To investigate the effect and mechanism of advanced oxidation protein products (AOPPs) on the apoptosis of HaCaT cells. Methods AOPPs were prepared by incubation of human serum albumin (HSA) with sodium hypochlorite solution. MTT assay was used to determine the effects of different concentrations (0, 50, 100, 200 and 400µg/ml) of AOPPs on HaCaT cell activity by incubating the cells for 24h for selecting optimum working concentration of AOPPs. HaCaT cells were treated based on time effect or concentration effect. The incidence of cell apoptosis was measured by flow cytometry. Intracellular reactive oxygen species (ROS) production was determined with dichlorofluorescein diacetate (DCFH-DA). And the protein expressions of NOX4, Bax and Bcl-2 were examined by Western blotting. Results Viability of HaCaT cells treated with 400µg/ml AOPPs was significantly lowered (P