Effects of PtoWOX11/12a Gene from Populus tomentosa on the Growth and Development of Cutting Seedlings in Poplar

【Objective】 WOX transcription factor family is one of the plant-specific transcription factor families,which have been demonstrated playing an important role in the embryonic patterning,stem cell maintenance and proliferation and lateral organ development. This study analyzed the roles of PtoWOX11/12 a gene from Populus tomentosa in the development of leaves and stems of transgenic 84 K poplar( Populus alba × P. glandulosa cl. 84 K). The gene expression level during the process of adventitious roots formation of Populus tomentosa,and in overexpression and suppressed expression of PtoWOX11/12 a transgenic 84 K poplars were analyzed. Our research provides a foundation for further studies of the roles of WOXs gene in plant development.【Method】 The leaf shape,height and basal diameter of one-month-old tothree-month-old transgenic lines and non-transgenic 84 K poplar( control) in the greenhouse were measured and compared. The anatomy of stem sections of the 5 th,9 thand 13 thinternodes of transgenic lines and cont