Overview of the improvement of the ring-stage survival assay - a novel phenotypic assay for the detection of artemisinin-resistant Plasmodium falciparum

Artemisinin resistance in Plasmodium falciparum threatens the remarkable efficacy of artemisininbased combination therapies worldwide. Thus, greater insight into the resistance mechanism using monitoring tools is essential. The ring-stage survival assay is used for phenotyping artemisinin-resistance or decreased artemisinin sensitivity. Here, we review the progress of this measurement assay and explore its limitations and potential applications.