Analysis and testing of bisphenol A, bisphenol A diglycidyl ether and their derivatives in canned dog foods

An analytical method was developed for the simultaneous determination of bisphenol A (BPA), bisphenol A diglycidyl ether (BADGE) and their derivatives, BADGE·2H 2 O, BADGE·H 2 O, BADGE·HCl·H 2 O, BADGE·2HCl and BADGE·HCl, in canned animal food commodities using reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection. The samples were cleaned up using solid-phase extraction (SPE) with hydrophobic polystyrene–divinylbenzene (PS/DVB) copolymer sorbent. The performance characteristics confirmed that the method used was in accordance with the EU validation criteria. The recovery values ranged from 62 to 97%. The repeatability (CV r ) and within-laboratory reproducibility (CV W ) values ranged from 2.3 to 9.7% and from 2.8 to 10.8%, respectively, while the limit of detection (LOD) and the limit of quantification (LOQ) values ranged from 5 to 10 µg/kg and from 5 to 15 µg/kg, respectively. The method was successfully used to evaluate the levels of 7 bisphenols in 24 of the canned dog foods retailed on the Slovenian market. BADGE·2H 2 O was found in all the samples tested, with values ranging from 16 to 540 µg/kg, while BPA was found in 96% of the samples tested and ranged from