Molecular identification of clinical Candida isolates by simple and randomly amplified polymorphic DNA-PCR

Thirty yeast isolates were obtained from specimens taken from different patients (sputum, vaginal and oral swabs). The isolates were identified by phenotyping and molecular methods. Six of the isolates were Candida albicans , identified by germ tube and CHROMagar. Three isolates were identified by CHROMagar as C. krusei . The remaining (21) clinical yeast isolates included C. sphaerica , C. guilliermondii C. kefyr , C. famata , C. glabrata , C. parapsilosis and C. norvegensis identified by Vitek2 and further confirmed by PCR. RAPD-PCR was also used in an attempt to identify DNA “fingerprints” for specific Candida spp. The DNA fingerprints of all Candida spp. except for C. sphaerica were determined.