The Impact of IL-10 C-597A Gene Polymorphisms on Asthma Susceptibility

Usually, the environmental factors require genetic background to exert their effects, and this explains the variation in asthma incidence among population exposed to the same environmental circumstances [12]. [...]determining the genetic factors of asthma has the priority in order to recognize the most prone subjects. The exclusion criteria for both patients and control were any history of respiratory illness like chronic obstructive pulmonary disease (COPD), tuberculosis, pneumonia, or bronchitis, and any other comorbid illness such as diabetes mellitus, hypertension, or pregnant females. Blood Samples, DNA Extraction and Gene Amplification: From each subject, 5ml of blood was drawn from vein puncture in an EDTA tube. Similar to T allele in IL-4C590T, A allele of IL-10 C-597A increases the accessibility of STAT 3 to its site in the promoter region but in contrast to IL-4-T590, IL-10 - A 597 has negative regulation activity. [...]allele A is associated with reduced production of IL-10 [23].