Expression and Response Analysis of NCED Gene of Zoysia japonica in Initial Infection Stage of Rhizoctonia solani

[Result] The expression level of NCED gene in stress treatment was higher than that without infection, which first increased then decreased with the extension of time, and reached the maximum at 36 h. Further detection of ABA level in 'Zenith' roots showed that the change trend of ABA level within 0 - 48 h was consistent with the expression pattern of NCED gene, and flattened out after 48 h. [ Conclusion] NCED gene regulates the synthesis of phytohormone ABA and probably further involves in plant disease resistance. Key words Zoysia japonica Steud. ; Rhizoctonia solani; NCED gene ; Quantitative expression Zoysia japonka Steud, , a kind of perennial warm-season herb with numerous excellent features, is widely applied in golf course, courtyard, playground and park, and it is extensively cultivated in subtropical region of China, with outstanding resistance to drought, high temperature and salt injury111. PCR results demonstrated that the amplification curves of NCED gene nearly gathered in a range at different infection time of R. solani; the melting curve had specific unimodal image at 79.62°C , while no obvious protrusion was observed at other temperatures, indicating PCR amplification products had strong specificity and fluorescence quantitative data were accurate ( Fig. 3 ). According to sequencing annotation results of transeriptome , the NCED gene of Z, japónica was screened, and sequenced by ordinary FCR.