[gamma]-Synuclein Is an Adipocyte-Neuron Gene Coordinately Expressed with Leptin and Increased in Human Obesity1-3

Recently, we characterized tumor suppressor candidate 5 (Tusc5) as an adipocyte-neuron PPARγ target gene. Our objective herein was to identify additional genes that display distinctly high expression in fat and neurons, because such a pattern could signal previously uncharacterized functional pathwa...

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Veröffentlicht in:The Journal of nutrition 2008-05, Vol.138 (5), p.841
Hauptverfasser: Oort, Pieter J, Knotts, Trina A, Grino, Michel, Naour, Nadia, Bastard, Jean-Phillipe, Clément, Karine, Ninkina, Natalia, Buchman, Vladimir L, Permana, Paska A, Luo, Xunyi, Pan, Guohua, Dunn, Tamara N, Adams, Sean H
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Sprache:eng
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Zusammenfassung:Recently, we characterized tumor suppressor candidate 5 (Tusc5) as an adipocyte-neuron PPARγ target gene. Our objective herein was to identify additional genes that display distinctly high expression in fat and neurons, because such a pattern could signal previously uncharacterized functional pathways shared in these disparate tissues. γ-Sy nuclein, a marker of peripheral and select central nervous system neurons, was strongly expressed in white adipose tissue (WAT) and peripheral nervous system ganglia using bioinformatics and quantitative PCR approaches. γ-Synuclein expression was determined during adipogenesis and in subcutaneous (SC) and visceral adipose tissue (VAT) from obese and nonobese humans. γ-Synuclein mRNA increased from trace levels in preadipocytes to high levels in mature 3T3-L1 adipocytes and decreased ~50% following treatment with the PPARγ agonist GW1929 (P < 0.01 ). Because γ-synuclein limits growth arrest and is implicated in cancer progression in nonadipocytes, we suspected that expression would be increased in situations where WAT plasticity/adipocyte turnover are engaged. Consistent with this postulate, human WAT γ-synuclein mRNA levels consistently increased in obesity and were higher in SC than in VAT; i.e. they increased ~1-7-fold in obese Pima Indian adipocytes (P = 0.003) and ~2-fold in SC and VAT of other obese cohorts relative to nonobese subjects. Expression correlated with leptin transcript levels in human SC and VAT (r = 0.887; P< 0.0001; n = 44). γ-Synuclein protein was observed in rodent and human WAT but not in negative control liver. These results are consistent with the hypothesis that y-synuclein plays an important role in adipocyte physiology. [PUBLICATION ABSTRACT]
ISSN:0022-3166
1541-6100