Effect of Thidiazuron on Adventitious Shoot Regeneration from Seedling Explants of Nothapodytes foetida

Regeneration of adventitious shoots from the medicinal plant Nothapodytes foetida (Weight) Sleumer Syn. Mappia foetida (family Icacinaceae) has been achieved using different seedling explants. Direct regeneration of shoot buds was observed in Murashige and Skoog's (MS) basal medium supplemented with various concentrations of thidiazuron. The optimum levels of thidiazuron concentrations were$0.91-4.45 \mu M$. Leaf explants formed more shoots followed by hypocotyls or cotyledons. The shoot buds elongated and rooted on MS basal medium with$N^6-benzyladenine (0.88-2.22 \mu M)$and indole-3-butyric acid ($0.49 \mu M$).