A comparative study of in vitro contractility between gut tissues of Hirschsprung's disease and other gut malformations

[...]it is also not clear yet, how various neurohumoral agents regulating the contractions are ineffective in regulating the motility in various gut malformations. [...]our question was whether such contractility regulatory mechanisms demonstrate same or different grade of activity in various obstructive gut malformations. [...]the time matched acetylcholine (100 pM)-induced amplitude of contractions before and after exposure to atropine (10 pM) of Hirschsprung's disease (H) were statistically compared with their corresponding values of non-Hirschsprung's cases (before values of H were compared with before values of NH and after values of H were compared with after values of NH). [15,22,26] In our observation, pheniramine (H1 blocker) failed to block histamine-induced gut muscle contraction in vitro in both the groups (Figure 4). [...]our observations do not support the involvement of H1 receptor in mediating the gut smooth muscle contraction. [28] In an observation, it was concluded that H1, H2, H4 receptors are expressed in human gastrointestinal tract while H3 receptors were absent)291 Thus, above evidence and findings of the present observation suggest the operation of a non-H1, non-H2 and non-H3 histaminergic population of receptors responsible for producing gut contractility in this study. [...]involvement of H4 receptor or any other receptor may be speculated to be present in human gastrointestinal tract causing smooth muscle contraction.