In vitro hemolysis and lipid peroxidation-inducing activity of the tentacle extract of the sea anemone (Paracondylactis indicus Dave) in rat erythrocytes
Objective : In vitro hemolytic activity of the tentacle extract of Paracondylactis indicus (Dave), a sea anemone found in the eastern coastal region of West Bengal (India), was determined in rat erythrocytes. Materials and Methods : Acute toxicity study was carried out with the tentacle extract foll...
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Veröffentlicht in: | Indian journal of pharmacology 2007-05, Vol.39 (3), p.155 |
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Zusammenfassung: | Objective : In vitro hemolytic activity of the tentacle extract of
Paracondylactis indicus (Dave), a sea anemone found in the eastern
coastal region of West Bengal (India), was determined in rat
erythrocytes. Materials and Methods : Acute toxicity study was carried
out with the tentacle extract followed by detailed biochemical studies
to evaluate the direct and indirect hemolytic activities of the
extract. The effects of pH, temperature, divalent cations and chelating
agents on hemolysis were also evaluated. Efforts were also made to
elucidate the mechanism of the hemolytic activity of the extract.
Results : The tentacle extract of P. indicus (Dave) produced
significant hemolysis (both direct and indirect) in washed rat
erythrocytes. The direct hemolytic activity of the tentacle extract was
found to be both temperature- and pH-dependent. Divalent cations (Ca 2+
, Mg 2+ ) produced significant enhancement of direct hemolytic
activity. Hemolysis (both direct and indirect) was significantly
diminished in the presence of Zn 2+ , EDTA and p-bromophenacyl bromide.
The tentacle extract was found to induce lipid peroxidation in washed
erythrocytes, which was inhibited by chlorpromazine. The tentacle
extract demonstrated significant proteolytic activity, which was found
to decrease in the presence of protease inhibitors (EDTA, EGTA and
PMSF). Conclusion : P. indica tentacle extract (and in particular, the
60% cut fraction) probably contains enzymatic components like
phospholipase(s) along with proteases, which may be the contributing
factors for its observed hemolytic activity. |
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ISSN: | 0253-7613 1998-3751 |
DOI: | 10.4103/0253-7613.33436 |