ABA Biosynthesis Genes are Down-regulated While Auxin and Cytokinin Biosynthesis Genes are Up-regulated During the Release of Grapevine Buds From Endodormancy
Auxin–cytokinin (CK) interactions have been extensively studied in the control of bud outgrowth in herbaceous plants. However, in temperate woody plants where the meristem of dormant buds can be repressed by either exogenous or endogenous factors, abscisic acid (ABA) has been suggested as a potentia...
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Veröffentlicht in: | Journal of plant growth regulation 2017-12, Vol.36 (4), p.814-823 |
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Sprache: | eng |
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Zusammenfassung: | Auxin–cytokinin (CK) interactions have been extensively studied in the control of bud outgrowth in herbaceous plants. However, in temperate woody plants where the meristem of dormant buds can be repressed by either exogenous or endogenous factors, abscisic acid (ABA) has been suggested as a potential regulator of bud outgrowth. To investigate the involvement of ABA, CK, and auxin on bud sprouting in
Vitis vinifera
, single-bud cuttings were used under forced conditions. This artificial bud sprouting system mimics and hastens the natural sprouting process that occurs in spring. Our results showed that expression of the ABA biosynthesis gene
VvNCED1
decreased during incubation, whereas expression of the ABA catabolism gene
VvA8H3
remained unaltered. Expression of CK biosynthesis-related genes ISOPENTENYL TRANSFERASE (
VvIPTs
) and LONELY GUY (
VvLOG1
), CK catabolism-related gene CYTOKININ OXIDASE (
VvCKX3
), and key auxin biosynthesis gene
VvYUC3
increased with incubation time. Moreover, treatment with hydrogen cyanamide (HC), a compound that breaks vine latency, increased expression of
VvIPTs
and
VvLOG1
and reduced expression of
VvCKX3
and
VvNCED1
. These results are consistent with previous reports indicating that HC increases CK levels and decreases ABA levels in grapevine buds. Taken together, the results suggest that in the vine, bud sprouting is preceded by a decrease in ABA content and an increase in CK and auxin levels. |
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ISSN: | 0721-7595 1435-8107 |
DOI: | 10.1007/s00344-017-9685-7 |