The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice
The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of OsGA20ox2 by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed Agroba...
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| Veröffentlicht in: | Plant molecular biology reporter 2011-12, Vol.29 (4), p.952-960 |
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| creator | Qiao, Feng Zhao, Kai-Jun |
| description | The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of
OsGA20ox2
by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed
Agrobacterium
-mediated transformation of rice varieties QX1 and Zhongzuo0201. The RNAi transgenic lines derived from pCH1CK and pCH12CK demonstrated a clear reduction in height compared with the control plants (
P
|
| doi_str_mv | 10.1007/s11105-011-0309-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1956087689</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1956087689</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-ad1ec4b462892803aea7806416040d6541802bdfc223366318e78f3680eb01f23</originalsourceid><addsrcrecordid>eNp1kEFLw0AQhRdRsFZ_gLcFz6szu8lmcyxF20KxUup52aaTNKVu6m4K-u9NiAcvngYe33sDH2P3CI8IkD1FRIRUAKIABbmQF2yEaSZFbtBcshFkKhW5TuCa3cR4gK4DxozYYrMnvvDl8Uy-IN6UfP06qfnGhYra2ld9soqziYTmS_IZ-Y7x_O3ofMvnVFf7lteer-uCbtlV6Y6R7n7vmL2_PG-mc7FczRbTyVIUCnUr3A6pSLaJliaXBpQjlxnQCWpIYKfTBA3I7a4spFRKa4WGMlMqbYC2gKVUY_Yw7J5C83mm2NpDcw6-e2kxTzWYTJu8o3CgitDEGKi0p1B_uPBtEWxvzA7GbGfM9sZsvyyHTuxYX1H4s_xv6QdkPWmo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1956087689</pqid></control><display><type>article</type><title>The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice</title><source>SpringerNature Journals</source><creator>Qiao, Feng ; Zhao, Kai-Jun</creator><creatorcontrib>Qiao, Feng ; Zhao, Kai-Jun</creatorcontrib><description>The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of
OsGA20ox2
by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed
Agrobacterium
-mediated transformation of rice varieties QX1 and Zhongzuo0201. The RNAi transgenic lines derived from pCH1CK and pCH12CK demonstrated a clear reduction in height compared with the control plants (
P
< 0.01). The height of dwarf lines from pCH1CK and pCH12CK were about 75–84% and 54–74% of that of the control plants, respectively. Transcripts for semi-dwarf lines from pCH1CK and pCH12CK were also reduced in comparison with the control plants. Analyses of seeds per panicle, 1,000-grain weight, and seed yield per plant suggested that the RNAi semi-dwarf lines showed stable grain yield compared with the wild-type plants. We further showed that the RNAi semi-dwarf lines could be restored to normal plant height by applying exogenous GA
3
. The results also indicate that different fragment of the same RNAi target can be practically applied to produce different extent semi-dwarf of rice plants.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-011-0309-2</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Bioinformatics ; Biomedical and Life Sciences ; Biosynthesis ; Crop yield ; Double-stranded RNA ; Gene expression ; Genetic transformation ; Grain ; Life Sciences ; Metabolomics ; Oryza ; Plant Breeding/Biotechnology ; Plant Sciences ; Plants (botany) ; Proteomics ; Ribonucleic acid ; Rice ; RNA ; RNA-mediated interference ; Seeds</subject><ispartof>Plant molecular biology reporter, 2011-12, Vol.29 (4), p.952-960</ispartof><rights>Springer-Verlag 2011</rights><rights>Plant Molecular Biology Reporter is a copyright of Springer, 2011.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-ad1ec4b462892803aea7806416040d6541802bdfc223366318e78f3680eb01f23</citedby><cites>FETCH-LOGICAL-c316t-ad1ec4b462892803aea7806416040d6541802bdfc223366318e78f3680eb01f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11105-011-0309-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11105-011-0309-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Qiao, Feng</creatorcontrib><creatorcontrib>Zhao, Kai-Jun</creatorcontrib><title>The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of
OsGA20ox2
by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed
Agrobacterium
-mediated transformation of rice varieties QX1 and Zhongzuo0201. The RNAi transgenic lines derived from pCH1CK and pCH12CK demonstrated a clear reduction in height compared with the control plants (
P
< 0.01). The height of dwarf lines from pCH1CK and pCH12CK were about 75–84% and 54–74% of that of the control plants, respectively. Transcripts for semi-dwarf lines from pCH1CK and pCH12CK were also reduced in comparison with the control plants. Analyses of seeds per panicle, 1,000-grain weight, and seed yield per plant suggested that the RNAi semi-dwarf lines showed stable grain yield compared with the wild-type plants. We further showed that the RNAi semi-dwarf lines could be restored to normal plant height by applying exogenous GA
3
. The results also indicate that different fragment of the same RNAi target can be practically applied to produce different extent semi-dwarf of rice plants.</description><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Crop yield</subject><subject>Double-stranded RNA</subject><subject>Gene expression</subject><subject>Genetic transformation</subject><subject>Grain</subject><subject>Life Sciences</subject><subject>Metabolomics</subject><subject>Oryza</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>Plants (botany)</subject><subject>Proteomics</subject><subject>Ribonucleic acid</subject><subject>Rice</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Seeds</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kEFLw0AQhRdRsFZ_gLcFz6szu8lmcyxF20KxUup52aaTNKVu6m4K-u9NiAcvngYe33sDH2P3CI8IkD1FRIRUAKIABbmQF2yEaSZFbtBcshFkKhW5TuCa3cR4gK4DxozYYrMnvvDl8Uy-IN6UfP06qfnGhYra2ld9soqziYTmS_IZ-Y7x_O3ofMvnVFf7lteer-uCbtlV6Y6R7n7vmL2_PG-mc7FczRbTyVIUCnUr3A6pSLaJliaXBpQjlxnQCWpIYKfTBA3I7a4spFRKa4WGMlMqbYC2gKVUY_Yw7J5C83mm2NpDcw6-e2kxTzWYTJu8o3CgitDEGKi0p1B_uPBtEWxvzA7GbGfM9sZsvyyHTuxYX1H4s_xv6QdkPWmo</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Qiao, Feng</creator><creator>Zhao, Kai-Jun</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20111201</creationdate><title>The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice</title><author>Qiao, Feng ; Zhao, Kai-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-ad1ec4b462892803aea7806416040d6541802bdfc223366318e78f3680eb01f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Crop yield</topic><topic>Double-stranded RNA</topic><topic>Gene expression</topic><topic>Genetic transformation</topic><topic>Grain</topic><topic>Life Sciences</topic><topic>Metabolomics</topic><topic>Oryza</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Sciences</topic><topic>Plants (botany)</topic><topic>Proteomics</topic><topic>Ribonucleic acid</topic><topic>Rice</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Seeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiao, Feng</creatorcontrib><creatorcontrib>Zhao, Kai-Jun</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiao, Feng</au><au>Zhao, Kai-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2011-12-01</date><risdate>2011</risdate><volume>29</volume><issue>4</issue><spage>952</spage><epage>960</epage><pages>952-960</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of
OsGA20ox2
by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed
Agrobacterium
-mediated transformation of rice varieties QX1 and Zhongzuo0201. The RNAi transgenic lines derived from pCH1CK and pCH12CK demonstrated a clear reduction in height compared with the control plants (
P
< 0.01). The height of dwarf lines from pCH1CK and pCH12CK were about 75–84% and 54–74% of that of the control plants, respectively. Transcripts for semi-dwarf lines from pCH1CK and pCH12CK were also reduced in comparison with the control plants. Analyses of seeds per panicle, 1,000-grain weight, and seed yield per plant suggested that the RNAi semi-dwarf lines showed stable grain yield compared with the wild-type plants. We further showed that the RNAi semi-dwarf lines could be restored to normal plant height by applying exogenous GA
3
. The results also indicate that different fragment of the same RNAi target can be practically applied to produce different extent semi-dwarf of rice plants.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><doi>10.1007/s11105-011-0309-2</doi><tpages>9</tpages></addata></record> |
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| subjects | Bioinformatics Biomedical and Life Sciences Biosynthesis Crop yield Double-stranded RNA Gene expression Genetic transformation Grain Life Sciences Metabolomics Oryza Plant Breeding/Biotechnology Plant Sciences Plants (botany) Proteomics Ribonucleic acid Rice RNA RNA-mediated interference Seeds |
| title | The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice |
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