The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice

The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice

The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of OsGA20ox2 by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed Agroba...

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Veröffentlicht in:Plant molecular biology reporter 2011-12, Vol.29 (4), p.952-960
Hauptverfasser: Qiao, Feng, Zhao, Kai-Jun
Format: Artikel
Sprache:eng
Schlagworte:
Bioinformatics
Biomedical and Life Sciences
Biosynthesis
Crop yield
Double-stranded RNA
Gene expression
Genetic transformation
Grain
Life Sciences
Metabolomics
Oryza
Plant Breeding/Biotechnology
Plant Sciences
Plants (botany)
Proteomics
Ribonucleic acid
Rice
RNA
RNA-mediated interference
Seeds
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Zusammenfassung:The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of OsGA20ox2 by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed Agrobacterium -mediated transformation of rice varieties QX1 and Zhongzuo0201. The RNAi transgenic lines derived from pCH1CK and pCH12CK demonstrated a clear reduction in height compared with the control plants ( P  
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-011-0309-2