A rapid and efficient DNA minipreparation suitable for screening transgenic plants

A rapid and efficient DNA minipreparation suitable for screening transgenic plants

We present a modified method for DNA minipreparation suitable for large-scale screening of transgenic plants. The method is rapid and efficient--one person can prepare DNA from approximately 50 samples per day. The average yield was about 40 μg DNA per 100 mg of fresh tissue, and the A^sub 260^/A^su...

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Veröffentlicht in:Plant molecular biology reporter 2001-12, Vol.19 (4), p.379-379
Hauptverfasser: Lin, Rong-Cheng, Ding, Zai-Song, Li, Liang-Bi, Kuang, Ting-Yun
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyribonucleic acid
DNA
Screening
Southern blotting
Transgenic plants
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Zusammenfassung:We present a modified method for DNA minipreparation suitable for large-scale screening of transgenic plants. The method is rapid and efficient--one person can prepare DNA from approximately 50 samples per day. The average yield was about 40 μg DNA per 100 mg of fresh tissue, and the A^sub 260^/A^sub 280^ was 1.89-2.03. The total DNA extracted by this method could be used for PCR, restriction enzyme digestion, and Southern blotting.
ISSN:0735-9640
1572-9818
DOI:10.1007/BF02772839