Mammalian LIN-7 PDZ proteins associate with [beta]-catenin at the cell-cell junctions of epithelia and neurons

The heterotrimeric PDZ complex containing LIN-2, LIN-7 and LIN-10 is known to be involved in the organization of epithelial and neuronal junctions in Caenorhabditis elegans and mammals. We report here that mammalian LIN-7 PDZ proteins form a complex with cadherin and [beta]-catenin in epithelia and neurons. The association of LIN-7 with cadherin and [beta]-catenin is Ca2+ dependent and is mediated by the direct binding of LIN-7 to the C-terminal PDZ target sequence of [beta]-catenin, as demonstrated by means of co-immunoprecipitation experiments and in vitro binding assays with the recombinant glutathione S-transferase:LIN-7A. The presence of [beta]-catenin at the junction is required in order to relocate LIN-7 from the cytosol to cadherin-mediated adhesions, thus indicating that LIN-7 junctional recruitment is [beta]-catenin dependent and that one functional role of the binding is to localize LIN-7. Moreover, when LIN-7 is present at the [beta]-catenin-containing junctions, it determines the accumulation of binding partners, thus suggesting the mechanism by which [beta]-catenin mediates the organization of the junctional domain.