ESTABLISHMENT OF AN IMPROVED, EFFICIENT AND ECO-FRIENDLY MICROPROPAGATION SYSTEM IN SALACIA CHINENSIS L. AN ENDANGERED ANTI-DIABETIC MEDICINAL PLANT

MATERIALS AND METHODS Indirect organogenesis Plant material, surface sterilization and culture conditions Young and healthy shoot tips, nodal segments and leaves collected from field grown S. chinensis were washed twice in running tap water, pre-washed with concentrated dishwasher gel (3-4 drops/100 ml double-distilled water (ddH2O) (Vim, India) for 5 min. According to data among various kind and concentration of auxins individually tested, the maximum callus (51.80 ± 0.70%) was induced on MS medium fortified by 1.0 mg/l NAA in nodal explants (Table 1) after 8 weeks. Jayaraman, S., Daud, N., Halis, R.& Mohamed, R. (2014): Effects of plant growth regulators, carbon sources and pH values on callus induction in Aquilaria malaccensis leaf explants and characteristics of the resultant calli. Kishino, E., Ito, T., Fujita, K.& Kiuchi, Y. (2009): A mixture of Salacia reticulata (Kotala himbutu) aqueous extract and cyclodextrin reduces body weight gain, visceral fat accumulation, and total cholesterol and insulin increases in male Wistar fatty rats.