Development of an approach to effective designing of fluorescent probes for real-time PCR

Development of an approach to effective designing of fluorescent probes for real-time PCR

A method is developed for creating fluorescent-labeled probes for real-time polymerase chain reaction for the purpose of a quantitative diagnosis of transgenic plants. Methods enabling designing of probes with a minimum initial level of the fluorescent signal are examined and analyzed.[PUBLICATION A...

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Veröffentlicht in:Russian agricultural sciences 2007-12, Vol.33 (6), p.356-360
Hauptverfasser: Gudov, V. P., Alekseev, Ya. I., Varlamov, D. A., Lunin, V. G., Kharchenko, P. N.
Format: Artikel
Sprache:eng
Schlagworte:
Fluorescence
Polymerase chain reaction
Probes
Studies
Transgenic plants
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Zusammenfassung:A method is developed for creating fluorescent-labeled probes for real-time polymerase chain reaction for the purpose of a quantitative diagnosis of transgenic plants. Methods enabling designing of probes with a minimum initial level of the fluorescent signal are examined and analyzed.[PUBLICATION ABSTRACT]
ISSN:1068-3674
1934-8037
DOI:10.3103/S106836740706002X