Analytical Methods for Pectin Methylesterase Activity Determination: a Review

Pectin methylesterase is an enzyme with an important in vivo role in plants as well as in food industry. This enzyme catalyzes the hydrolysis of methyl ester bounds in pectin which is one of the main components of cell wall in plants, producing methanol and free carboxylic groups. The effect of pect...

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Veröffentlicht in:Food analytical methods 2017-11, Vol.10 (11), p.3634-3646
Hauptverfasser: Salas-Tovar, Jesús A., Flores-Gallegos, Adriana C., Contreras-Esquivel, Juan C., Escobedo-García, S., Morlett-Chávez, Jesús A., Rodríguez-Herrera, Raúl
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container_issue 11
container_start_page 3634
container_title Food analytical methods
container_volume 10
creator Salas-Tovar, Jesús A.
Flores-Gallegos, Adriana C.
Contreras-Esquivel, Juan C.
Escobedo-García, S.
Morlett-Chávez, Jesús A.
Rodríguez-Herrera, Raúl
description Pectin methylesterase is an enzyme with an important in vivo role in plants as well as in food industry. This enzyme catalyzes the hydrolysis of methyl ester bounds in pectin which is one of the main components of cell wall in plants, producing methanol and free carboxylic groups. The effect of pectin methylesterase in food quality has been extensively studied, producing desirable effects in texture improvement as well as undesirable effects in some beverages. Likewise, the low methoxyl pectin produced by this enzyme has characteristics that contribute to formulate best quality food products. Pectin methylesterase is a ubiquitously enzyme that presents multiple isoforms, but is not only present in plants; it is also found in fungi, bacteria, and yeast, which have specific chemical and physical characteristics. The latter makes the task of analyzing the wide variety of these enzymes with its specific characteristics difficult. Based on this enzyme relevance and the aforementioned, multiple methods have been developed in order to evaluate pectin methylesterase activity with different research objectives. In this paper, the importance of the enzyme as well as advantages and drawbacks of the different methods will be discussed besides applications and evolution of these will be mentioned. Additionally, this paper will improve the understanding of the systems used in pectin methylesterase activity analysis.
doi_str_mv 10.1007/s12161-017-0934-y
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Based on this enzyme relevance and the aforementioned, multiple methods have been developed in order to evaluate pectin methylesterase activity with different research objectives. In this paper, the importance of the enzyme as well as advantages and drawbacks of the different methods will be discussed besides applications and evolution of these will be mentioned. 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subjects Analytical Chemistry
Analytical methods
Bacteria
Beverages
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Enzymes
Food
Food industry
Food plants
Food processing industry
Food production
Food quality
Food Science
Freezing
Fungi
Isoforms
Microbiology
Pectin
Pectinesterase
Physical characteristics
Physical properties
Yeast
title Analytical Methods for Pectin Methylesterase Activity Determination: a Review
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