Hydrocarbonoclastic bacteria of the genus Pseudomonas in Samanea saman (Jacq.) Merr. rhizosphere

The research goal includes isolation, characterization and identification of Pseudomonas species existing in the rhizosphere of a legume present (colonizing or survivor) in a savanna soil polluted by an oil spill, in order to explain the support of the growth of this leguminous plant through the reduction of the toxicity of spilled oil (hydrocarbonoclastic effects). The site is located at Amana del Tamarindo village entrance, Monagas State, Venezuela (9 ° 38' 52 "N, 63 ° 7' 20" E, 46 masl). An area of 50 m2 was sampled. In concordance to the descriptions, keys, and comparison with the UOJ Herbarium exsiccatae, the legume collected was identified as Samanea saman (Jacq.) Merr., which belongs to the Fabaceae family. The results of the biochemical characterization and the production of pyocyanine and fluorescein pigments allowed identifying 10 isolates as P. fluorescens, 5 as P. putida and 5 as P. aeruginosa. Samanea saman is recommended for re-vegetation of the contaminated area.