Piezoelectric biosensor for detection of Salmonella typhimurium

A flow injection analysis (FIA) system was developed based on a piezoelectric biosensor for detection of Salmonella typhimurium. The anti-Salmonella spp antibody was immobilized onto the gold electrode coated quartz crystal surface through a polyethylenimine-glutaraldehyde (PEG) technique and dithio...

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Veröffentlicht in:	Journal of food science 1997-09, Vol.62 (5), p.1067-1086
Hauptverfasser:	Ye, J. (Univ. of Rhode Island, West Kingston, RI.), Letcher, S.V, Rand, A.G
Format:	Artikel
Sprache:	eng
Schlagworte:	ANTIBODIES antibody immobilization ANTICORPS ANTICUERPOS BIODETECTEUR Biological and medical sciences biosensor BIOSENSORES BIOSENSORS Biotechnology Food contamination & poisoning Food industries Food microbiology Food science Fundamental and applied biological sciences. Psychology IMMOBILISATION IMMOBILIZATION INMOVILIZACION Methods. Procedures. Technologies piezoelectric QCM Salmonella SALMONELLA TYPHIMURIUM Sensors Various methods and equipments
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Beschreibung
Zusammenfassung:	A flow injection analysis (FIA) system was developed based on a piezoelectric biosensor for detection of Salmonella typhimurium. The anti-Salmonella spp antibody was immobilized onto the gold electrode coated quartz crystal surface through a polyethylenimine-glutaraldehyde (PEG) technique and dithiobis-succinimidyl propionate (DSP) coupling. The PEG technique proved more successful for FIA applications than the DSP coupling. The biosensor had responses of 23-47 Hz in 25 min when the PEG immobilization technique was employed, with R20.94 for Salmonella typhimurium concentrations of 5.3 X 10(5) to 1.2 X 10(9) CFU/mL
ISSN:	0022-1147 1750-3841
DOI:	10.1111/j.1365-2621.1997.tb15039.x