The Effect of the Incubation Temperature on Morphological and Physical Parameters of Hypophthalmichthys molitrix Erythrocytes and Polymorphonuclear Leukocytes

The purpose of our research is to estimate the temperature influence on morphological and physical parameters of erythrocytes and polymorphonuclear white blood cells in silver carp Hypophthalmichthys molitrix under in vitro conditions. Adhesion of leukocytes is changed only at low incubation temperature. [...]decreasing incubation temperature to 5°C, in comparison with at temperature of 20°C, contributes to increase of the adhesion of PMNL by 31.4%. Given that the major factors, causing changes in the physical and physiological properties of the blood cells, are the properties of plasmolemma [13, 14], we assume that the decline in its elasticity after incubation of the silver carp erythrocytes occurs due to changes in microviscosity of the lipid bilayers, phase distribution lipid, microenvironment protein, protein-lipid interactions, and other features of the structural membrane organization [14], manifesting small folding on the surface. Morphometric parameters of red blood cells and polymorphonuclear leukocytes in silver carp are not changed after the incubation at low (5°C) and elevated (40°C) temperatures, in comparison with incubation at temperature of 20°C, except for the volume of white blood cells at a temperature of 40°C. 3.