In silico and In vitro Evaluation of Anti-urolithiatic Activity of Ethanolic Extract of Syzygium cumini Stem Bark

Estimation of Calcium oxalate by Nucleation assay (Turbidity method): The Spectrophotometric assay was used to determine the inhibitory activity of the extracts on the nucleation of calcium oxalate crystals. 100pl of 50 mM sodium oxalate solutions and 100pl of 4 mM calcium chloride to 0.5ml of human normal urine, both prepared in a buffer containing 0.5ml of 0.05 mM Tris buffer and 0.5ml of 0.15mM NaCl solution at 37°C and pH 6.5, adjusted to volume by adding 1.5ml of distilled water, hence crystallization is initiated. [...]potent docking of the ligand on all sites for the receptor to possibly attach are well prognosticated and docked in HEX. All the compounds have showed NEGATIVE VALUES i.e., each and every compounds have greater affinity towards the receptor. [...]these compounds can be effectively used in regulating the human calcium sensing receptor and among all these compounds, Delphinidin 3-gentiobioside showed a very high energy binding value of -342.67. [...]the in vitro studies have proven to show that the Ethanolic extract of Syzygium cumini Stem bark are very efficient in the treatment of Urolithiasis. [...]the study proves that the extract as well and the isolated compounds of Syzygium cumini can be effectively used to control as well as eliminate the kidney stone formation.