Influence of cementation level on the strength behaviour of bio-cemented sand

Microbially induced calcite precipitation (MICP) is used increasingly to improve the engineering properties of granular soils that are unsuitable for construction. This shows MICP technique significant advantages such as low energy consumption and environmentally friendly feature. The objective of the present study is to assess the strength behaviour of bio-cemented sand with varying cementation levels, and to provide an insight into the mechanism of MICP treatment. A series of isotropic consolidated undrained compression tests, calcite mass measurement and scanning electron microscopy tests were conducted. The experimental results show that the strength of bio-cemented sand depends heavily on the cementation level (or calcite content). The variations of strength parameters, i.e. effective friction angle φ ′ and effective cohesion c ′, with the increase in calcite content can be well evaluated by a linear function and an exponential function, respectively. Based on the precipitation mechanism of calcite crystals, bio-clogging and bio-cementation of calcite crystals are correlated to the amount of total calcite crystals and effective calcite crystals, respectively, and contributed to the improvement in the effective friction angle and effective cohesion of bio-cemented sand, separately.