Flat‐mount preparation of lens epithelium

Summary The lens capsule with its attached epithelium is a relatively accessible epithelial model system, suitable for various cell culture assays, histology, and histochemical assays. The optical requirements for imaging of lens capsule‐epithelium can be challenging, especially if the tissue is not flat. The lecture will cover various techniques for preparation of flat‐mount capsule‐epithelium, including differences among species, removal of cortical tissue, and fixation of the capsule‐epithelium to various base materials.