ИНДУЦИРОВАННЫЙ МУТАΓЕНЕЗ В КУЛЬТУРЕ ИЗОЛИРОВАННЫХ ТКАНЕЙ СЕНПОЛИИ

The paper studied the effects of colchicine on isolated tissue regenerative capacity of violet saintpaulia (Blue Dragon). We determined the optimal dose of the mutagen exposure time and to preserve cell viability and maximize the number of plants regenerated with modified genotype. We used leave fragments (5 × 5 mm) for cell lines. Isolated leaf explants were cultured on MS medium supplemented with benzylaminopurine (BAP) and α-naphthaleneacetic acid at a concentration of 3 and 4 uM respectively. As used chemical mutagen colchicine at concentrations of 10, 50, 80 and 100 mg / l. Colchicine treatment were carried out at three exposures (3, 5 and 10 days), after which the callus tissue was transferred to fresh medium without colchicine for further cultivation. Served as controls on the cultivation medium without colchicine result of the impact of high concentrations of colchicine and duration of exposure to the media explant with a mutagen is the process of callus formation, which underwent surviving meristematic cells. In tissue culture to move into specialized tissue divisible state is always necessary hormonal factors. Probably, in our case, was an inducer such colchicine at concentrations of 50 and 80 mg / l at 10 days exposure. On media II-10 and III-10 callus level ranged from 60 to 100%. When the concentration of colchicine 50 and 80 mg / L and 10 days of exposure there is a low level of direct regeneration and a high percentage of callus formation. Therefore, we can expect changes in the genome of regenerated plants obtained on a medium II-10 and III-10.