Potential of using immobilizing agents in aided phytostabilization on simulated contamination of soil with lead

[Display omitted] Lead (Pb) is one of the key heavy metals which have a significant influence on the individual components of the natural environment. A glasshouse pot experiment was designed to evaluate the potential use of different amendments as immobilizing agents in the aided phytostabilization of Pb-contaminated soil, using Lolium perenne L. The research aimed to determine the influence of Pb in doses of 0, 100, 200, 400 and 800mg/kg of soil, as well as diatomite, chalcedonite, dolomite, limestone, and activated carbon amendments on the content of trace elements in the above-ground parts and roots of L. perenne. The study utilised analysis of variance (ANOVA), principal component analysis (PCA) and Factor Analysis (FA). The content of trace elements in plants, pseudo-total and extracted by 0.01M CaCl2, were determined using the method of spectrophotometry. All of the investigated element contents in the tested parts of L. perenne were significantly different in the case of applying reactive amendments to the soil, as well as increasing concentrations of Pb. The greatest average above-ground biomass was observed when diatomite and chalcedonite were amended into the soil. Activated carbon, limestone and chalcedonite caused significant increases of Pb concentrations in the roots.