Mössbauer spectroscopic study of iron and cobalt metabolic transformations in cells of the bacterium Azospirillum brasilense Sp7

The transmission ( 57 Fe) and emission ( 57 Co) variants of Mössbauer spectroscopy are used to monitor metabolic transformations of 57 Fe III / 57 Fe II and 57 Co II in cells of the bacterium Azospirillum brasilense Sp7. (The spectra are measured at 80 K.) For 57 Co II , metabolic changes in the living cells are observed after 1 h. Two components of high-spin 57 Fe II are observed for the bacteria grown with 57 Fe III as a source of iron; the parameters for 57 Fe III correspond to ferritin-like components.