In vitro antibacterial effectiveness of three different dentin bonding systems against Streptococcus mutans and Enterococcus faecalis

Aims: This study aims to evaluate the antibacterial effect of three different bonding systems - G-Bond, Clearfil SE Bond and Prime and Bond - on Streptococcus mutans and Enterococcus faecalis in vitro. Materials and Methods: This study examined the antibacterial effectiveness of dentin bonding systems against S. mutans and E. faecalis. This study was carried out with the standardized inoculums with selective media and the materials assayed were straight away applied on the cultures. Discs of dentin bonding system (4 mm × 4 mm) were made, cured, washed and put on the bacterial inoculums for 24, 48, and 72 h and the growth inhibition was evaluated using a spectrophotometer. The dentin bonding agents evaluated in this study revealed different inhibitory effects. Statistical Analysis Used: Submitted to the analysis of variance. Results: Group A (G-Bond) has an inhibitory effect compared to Group B (Clearfil SE Bond) and Group C (Prime and Bond NT) at 24, 48, and 72 h against S. mutans. Group B (Clearfil SE Bond) has inhibitory effect at 24, 48, and 72 h compared to Group A (G-Bond) and Group C (Prime and Bond NT) against E. faecalis. Conclusions: This self-etching system G Bond and Clearfil SE Bond presented a greater inhibitory effect against S. mutans and E. faecalis than the conventional system, Prime and Bond did.