A67 BREAKDOWN: THE CELLULAR CONTRIBUTION TO PULMONARY HYPERTENSION: Dasatinib-Induced Pleural Effusion Is Associated With Increased Endothelial Permeability

A67 BREAKDOWN: THE CELLULAR CONTRIBUTION TO PULMONARY HYPERTENSION: Dasatinib-Induced Pleural Effusion Is Associated With Increased Endothelial Permeability

Methods: To test this hypothesis, we used primary cultures of human umbilical vein endothelial cells (HUVEC) and human pulmonary microvascular endothelial cells (P-EC) as well as rats for in vitro and in vivo experiments, respectively. Results: Dasatinib, but not imatinib, led to a rapid (3 hours) i...

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Veröffentlicht in:American journal of respiratory and critical care medicine 2017-01, Vol.195
Hauptverfasser: Phan, C, Jutant, E -M, Tu, L, Tamura, Y, Thuillet, R, Hiress, M Le, Fadel, E, Simmoneau, G, Seferian, A, Montani, D, Huertas, A, Noordegraaf, A Vonk, Humbert, M, Aman, J, Guignabert, C
Format: Artikel
Sprache:eng
Schlagworte:
Hypertension
Inhibitor drugs
Permeability
Rodents
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Zusammenfassung:Methods: To test this hypothesis, we used primary cultures of human umbilical vein endothelial cells (HUVEC) and human pulmonary microvascular endothelial cells (P-EC) as well as rats for in vitro and in vivo experiments, respectively. Results: Dasatinib, but not imatinib, led to a rapid (3 hours) increase in paracellular permeability of HUVEC and P-EC monolayers as reflected by decreased transendothelial electrical resistance (TEER), increased macromolecule passage, loss of VE-cadherin and ZO-1 from cell-cell junctions, and development of actin stress fibers.
ISSN:1073-449X
1535-4970