D27 I WANNA BE YOUR ... NOVEL MECHANISTIC INSIGHT INTO PULMONARY VASCULAR DISEASE: Mirna Cargo In Microvesicles Released From Hypoxic Pulmonary Arterial Endothelial Cells Induces Pulmonary Vascular Smooth Muscle Cell Proliferation And Pulmonary Hypertension

Rationale: Crosstalk between pulmonary arterial endothelial cells (PAEC) and pulmonary arterial smooth muscle cells (PASMC) plays an important role in the pathogenesis of pulmonary hypertension (PH). [...]we injected 8-week old C57BL/6 mice with DMSO control or GW4869, a drug that is known to block the generation of extracellular vesicles, and exposed these mice to normoxia or 10% hypoxia for 3 weeks, followed by measurement of right ventricular systolic pressure (RVSP), RV hypertrophy (RV/(LV+S) ratio), and pulmonary arterial wall thickness.